Abstract: Due to increase in drug resistance world wide, this study was undertaken to characterize mode of transmission of blaCTX-M among ESBL-producing E. coli isolates. ERIC-PCR was used to type the ESBL-producers strains. A search for blaCTX-M genes and integrons in the genomic and plasmid DNA of ESBL positive isolates was performed by PCR amplification. Cloning, sequencing of CTX-M gene was performed and submitted in gene bank (FJ997864, FJ997865, GQ174503, GQ145221 and GQ174504). Modelling and docking was performed by Autodoc . The ERIC typed isolates were screened for blaCTX-M, blaTEM, armA, rmtA and rmtB. PCR amplified blaCTX-M genes were cloned and sequenced. Five blaCTX-M-15, 2 rmtB, 2 blaTEM-1 and 13 Class1 integrons were detected. All the blaCTX-M-15 positive isolates, except one were clonally related. ‘Length of stay in NICU’ was found as the single independent risk factor. This study concludes that resistant markers were transferred through plasmids in the present setting. Male neonates who are colonized or infected by ESBL-producing E. coli might have a longer stay in NICU compared to their female counterparts.

Brief Biography of the Speaker:
Asad U Khan, male, molecular biologist, graduated from Biochemistry Department, A M University, Aligarh, India in 1998. He joined Interdisciplinary Biotechnology Unit, A.M University as Asst Professor in 1997 and continued till 2000. He later joined Department of Biochemistry, UMDNJ, New Jersey USA in 2000 for three years as post doctoral Research Associate, working on Transcription biology of Yeast and gene expression. He than resumed his services as Asst Professor in the same department till 2006 and became Associate professor in the Interdisciplinary Biotechnology Unit, AMU, India. He was awarded a prestigious fellowship , BOYSCAST Fellowship from Government of India to work as visiting Scientist in University of Napoli, Italy in 2005. He was also awarded Young Scientist Award of Association of Microbiologist of India in 2006 and Alembic award in 2009. His work was well recognized and he has been invited several invited talks as well as reviews articles and chapters. He has been in Editorial board of several number of International journal. He has a total of 85 research articles in his credit. He is a member of several international associations.