Abstract: Escherichia coli (E.coli) is a common bacteria that colonize digestive truck of many organisms including humans and is a important source of Vitamin K and B12. However, numerous strains of E.coli are pathogenic and cause severe infections and alimentary toxemia. Eight toxic strains of E.coli are known till now. The main source of E.coli infections is water. That is why monitoring the purity level of water sources is extremely important for public health. Commonly used methods for E.coli detection are time-consuming and rely on E.coli culturing and cells differentiation. Noticeably faster and more sensitive method for E.coli detection is a PCR where even small copy of E.coli genome might be detected. What is more, the analysis can be done in about one hour, what accelerate the time of analysis.
PCR (Polymerase Chain Reaction) is sensitive and specific tool for DNA fragments amplification and utility of this technique is still growing. Here we are presenting a device dedicated for DNA fragments amplification based on Real-Time PCR approach. We designed the system for E.coli DNA fragment amplification and detection and at the same time for E.coli quantification. The LTCC technology was used for structure fabrication. We successfully performed PCR, where we were able to amplify region of E.coli DNA. The miniaturized PCR system based on the LTCC technology might provides the possibility for faster and cheaper E.coli detection in a different source of environmental samples even by small laboratories or the one created in a place of potentially E.coli contamination.

Brief Biography of the Speaker:
Malgorzata Malodobra graduated from the Wroclaw Medical University in Poland in 2006. In the 2010 she received the Ph.D. title at Wroclaw Medical University in Molecular Technique Unit. From 2010 she works as a Assistance of Professor in Molecular Technique Unit at Wroclaw Medical University. In 2009 she performed an internship in Joslin Diabetes Center at Harvard Medical School, MA, USA. She is interested in the molecular basis of metabolism syndrome, insulin resistance and type 2 diabetes. She is also involved in many research project performed in Molecular Techniques Unit. From 2008 she works also as a specialist in research project dedicated to Electronic Microsystem development standing for a single used thermalcycler for PCR in a wide range of use, especially for medicine and environmental hazardous factors detection. She is also performing a diagnostic research of genetic disorders or a pathogens detection. She is an author or co-author of eight full text publication and an author or co-author of 13 conference abstracts in a wide field of molecular medicine research.